Coronaviruses: Methods and Protocols PDF Free

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    Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
    Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix

    1 Coronaviruses: An Overview of Their Replication and Pathogenesis . . . . . . . . 1
    Anthony R. Fehrand Stanley Perlman
    PART I DETECTION, DIAGNOSIS AND EVOLUTION OF CORONAVIRUSES
    2 Identification of a Novel Coronavirus from Guinea Fowl
    Using Metagenomics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
    Mariette F. Ducatezand Jean-Luc Guérin
    3 Serological Diagnosis of Feline Coronavirus Infection
    by Immunochromatographic Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
    Tomomi Takanoand Tsutomu Hohdatsu
    4 Estimation of Evolutionary Dynamics and Selection Pressure
    in Coronaviruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
    Muhammad Munirand Martí Cortey
    PART II PROPOGATION, TITRATION, AND PURIFICATION
    OF CORONAVIRUSES
    5 The Preparation of Chicken Tracheal Organ Cultures
    for Virus Isolation, Propagation, and Titration . . . . . . . . . . . . . . . . . . . . . . . . 51
    Ruth M. Hennion
    6 The Preparation of Chicken Kidney Cell Cultures for Virus Propagation . . . . . 57
    Ruth M. Hennionand Gillian Hill
    7 Isolation and Propagation of Coronaviruses in Embryonated Eggs . . . . . . . . . 63
    James S. Guy
    8 Characterization of Human Coronaviruses on Well- Differentiated
    Human Airway Epithelial Cell Cultures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
    Hulda R. Jonsdottirand Ronald Dijkman
    9 Quantification of Infectious Bronchitis Coronavirus
    by Titration In Vitro and In Ovo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
    Joeri Kint , Helena Jane Maier , and Erik Jagt
    10 Purification of Coronavirus Virions for Cryo-EM and Proteomic Analysis. . . . 99
    Stuart Dentand Benjamin W. Neuman
    11 Partial Purification of IBV and Subsequent Isolation of Viral RNA
    for Next-Generation Sequencing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
    Sarah M. Keep , Erica Bickerton , and Paul Britton
    Contents

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